Gunnersen Lab ~ Neuron Development and Plasticity Laboratory
Neurons from Sez-6 knockout mice have more dynamic filopodia
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Wildtype |
Sez6 knockout |
|---|---|
Dynamic properties of filopodia were measured according to Luikart et al. (2008). Briefly, filopodial turnover was measured as the average of the sum of new filopodia appearing and the number of filopodia eliminated per dendrite length every 5 min for a total of 60 min. This measurement was performed manually using Autotrace (Britto et al., 2011). Filopodial motility was calculated as the average of the absolute value of the sum of changes in filopodial lengths per dendrite length every 5 min for a total of 60 min. The length of each filopodia was measured by manually tracing using Autotrace.
- Britto JM, Tait K, Johnston LA, Hammond VE, Kalloniatis M, Tan SS (2011) Altered Speeds and Trajectories of Neurons Migrating in the Ventricular and Subventricular Zones of the Reeler Neocortex. Cerebral Cortex 21(5), 1018-1027.
- Luikart BW, Zhang W, Wayman GA, Kwon CH, Westbrook GL, Parada LF (2008) Neurotrophin-dependent dendritic filopodial motility: a convergence on PI3K signaling. J Neurosci. 28(27):7006-12.