Young Lab ~ Techniques
We use wild-type and a variety of transgenic and naturally occurring mutant mice and utilize the following techniques:
top of page1. Immunohistochemistry and in situ hybridization
These techniques enable us to determine the localization of molecules of interest.
2. Time lapse microscopy
This enables us to examine the cellular behaviour of living neural cells cells as they migrate through the gut and to determine how particular molecules affect migratory behaviour.
3. Cultured, intact explants of gut
These enable us to examine the roles of particular molecules on neural crest cell migration.
4. Collagen gel assay
This enables us to determine if particular molecules are chemoattractive or chemorepulsive to neural crest cells.
5. Spatiotemporal mapping
This enables the motility along the length of the gut to be analyzed over time.
